Aquacultura Indonesiana (2015) 16 (1) : 16-21

The Intracellular Cryoprotectant Effects in Preserving GoramyA�Spermatozoa after Two Days Sub-Zero FreezingA�

Abinawanto1, Nisa Fitrianingrum1, Retno Lestari1, Agung Sudaryono2, Rita Rostika3,A�and Yushinta Fujaya4

1 Biology Graduate Study Program, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia
2 Aquaculture Study Program, Diponegoro University, Semarang, Indonesia
3 Faculty of Fisheries and Marine Science, Padjadjaran University, Bandung, Indonesia
4Faculty od Marine Science and Fisheries, Hasanudin University, Makassar, Indonesia
Correspondence to: abinawanto.ms@sci.ui.ac.id

Abstract

A�The spermatozoa quality ofA�goramy after 2 d sub-zero freezing was examined. The quality of spermatozoa examined included motility,A�viability, and abnormality. We aimed to determine the optimum concentration of glycerol protectingA�spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively. Sperms wereA�diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute spermsA�were then equiliberated at 4A�C for 45 min, and were freezed at -34A�C for 2 d. Thawing was then carried out atA�30A�C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoaA�motility (75.95A�4.76)%.

Keywords: Glycerol; Osphronemus goramy; Spermatozoa motility; Sub-zero freezing; Viability andA�Abnormality

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